Fig 1: Tissue analyses reveal PRDM12 expression in adult mouse peripheral and central nervous tissue. (A) Upper panel, western blot analysis shows a slight PRDM12 protein expression in adult heart tissue, but no detectable expression in the lungs, livers, kidneys, or spleen. Lower panel, western blot indicates PRDM12 protein level expression in the central nervous system (CNS): whole brain (WB), cortex, hypothalamus (Hypo.), hippocampus (Hipp.), cerebellum (CBL), and spinal cord (SC), as well as in peripheral nervous system (PNS), and dorsal root ganglia (DRG). Antibody positive control (PRDM12WT) is HEK293 cell lysate overexpressing wild type (WT) PRDM12 complementary DNA (cDNA) and negative control (PRDM12S58fs) is HEK203 cell lysate overexpressing PRDM12S58fs cDNA. HSP90 was used as loading control. (B–E) Representative immunohistochemical images of adult mouse lumbar DRG sections indicating PRDM12 co-expression with classical nociceptive markers: IB4, Nav1.8, CGRP, and tyrosine kinase 1 (TRKA), as labeled. White arrows indicate cells with co-labeling, with digitally enlarged insets of select co-labeled cells. Scale bar in all images is 20 μm.
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